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KMID : 0378019750180010057
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New Medical Journal
1975 Volume.18 No. 1 p.57 ~ p.63
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A Study on the Survival of Lactobacilli and Shigella in Mouse Cecum
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Abstract
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The present study has been designed in order to clarify the multiplication and the effect of lactobacilli (shirota strain) on the growth of Shigella dysenteriae (type 1) in vitro and in vivo mouse cecum. The experimental results are summerized as follows.
1. The growth and multiplication of Shigella dysenteriae were inhibited when those organisms were inoculated on the L-broth medium culturedwith lactobacilli for 16 hours (pH 5-0). However, when Shigella dysenteriae was inoculated on the BHI broth medium already cultured with lactobacilli, -the growth and multiplication of those organism were observed, and pH of the medium was changed from 6.3 to 6.1
2. Whe both lactobacilli and Shigella dysenteriae were simultaneously inoculated on the L-broth or BHl broth medium, there were observed decreasing trends of survival of shigella organism, while lactobacilli gradualy grew and multiplied, and pH of the L-broth and BHI broth were changed from 6.8 to 5. 6 an
d from 7. 2 to 6.3 respectively.
3. There was no distinct growth and multiplication of lactobacilli in mouse cecum by oral administration, although there was a slight fluctuation in the number of lactobacilli organisms.
4. Shigella organisms was detected with gradual decreasing numbers untill the fourth day after inoculation by mouth. However the survial of Shigella organisms in mouse cecum was rapidly decreased and disappeared within one day by the oral administration of lactobacilli solution.
Based on the above experimental results it is deduced that lactobacilli are generally unable to be multiplicated in mouse cecum and to affect pH change of mouse cecum, and that shigella dysenteriae survival in mouse cecum seems not to be affected by pH fluctuation.
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